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Title: 3103 - Antibacterial Effect of ERRM® and ProRoot MTA against Bacterial Biofilm

Authors:

Mayra Téllez (Presenter)
Pontificia Universidad Javeriana

Angela Burbano, Universidad Santo Tomás
Andrés Carrillo, Universidad Santo Tomás
Yamile Guerra, Universidad Santo Tomás
Yerka Romero, Universidad Santo Tomás
Oscar Jiménez, Universidad Santo Tomás
Nelly Roa, Pontificia Universidad Javeriana
Angela Chaves, Universidad Santo Tomás

Abstract:

Objectives: To determine the antibacterial effect of the ERRM® root repair material compared to ProRoot White® (MTA) against in vitro bacterial biofilm model using Confocal Microscopy (CLSM), to propose an cement in perirradicular surgery able to avoid endodontic failure persist due to primary, secondary and persistent infections in the root canals and periradicular tissues, caused by microorganisms such as Enterococcus faecalis, Fusobacterium nucleatum, Streptococcus gordonii, Porphyromonas gingivalis, Actinomyces naeslundi that have shown resistance to chemical-mechanical and antibiotic therapies, by the ability to form biofilms.

Methods: Direct contact method was performed with a dilution of the materials at a concentration of 125 mg/mL, against in vitro biofilm model made on synthetic hydroxyapatite discs, incubated at 37 degrees celsius in anaerobic conditions and it was evaluated at 24, 48 and 72 hours. Confocal laser scanning microscopy was done to determinate bacterial biofilm viability. Kolmogorov-Smirnov test was performed to evaluate the normality of the data distribution; statistical significance was established when the p-value was less than 0.05.

Results: The percentage of dead bacteria of biofilm with ERRM® was 5%, 11% and 10% at 24, 48 and 72 hours, respectively, meaning that this effect was better at longer exposure time. In turn, the MTA showed percentages of 12%, 9% and 6% at 24, 48 and 72 hours respectively, which indicates that over time the antibacterial effect decreased. There were no differences.

Conclusions: An In vitro biofilm model was developed and neither of the two materials studied ERRM® root repair material and ProRoot White® (MTA) had antibacterial efficacy against the biofilm.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE

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