Title: 2044 - Cytotoxicity of Enamel Remineralizing Plant Extracts on Pulpal Stem Cells -


Sandra Gutierrez (Presenter)
Pontificia Universidad Javeriana

Luisa Suarez, Pontificia Universidad Javeriana
Henry Mendez, Pontificia Universidad Javeriana
luis Sequeda, Pontificia Universidad Javeriana
Juan Salcedo, Pontificia Universidad Javeriana


Objectives: Amelogenesis imperfecta (AI), affects the enamel structure, causing teeth sensitivity and susceptibility to caries. Currently, its treatment is based on the incorporation of minerals to remineralize the enamel. A previous research has shown that extracts from Colombian native plants such as Ilex guayusa and Piper marginatum appose minerals to the tooth enamel structure, but it is unknown if they produce toxic effects on the dental pulp.

Objective: To evaluate the cytotoxicity produced by two extracts of native Colombian plants (Ilex guayusa and Piper marginatum) on mesenchymal stem cells of human dental pulp (hDPSC) through Raman spectroscopy.

Methods: hDPSC were cultured on nano biochips placed on culture discs. After 8 days, concentrations of 10, 50 and 100 ppm, of each of the extracts were add separately on the discs. Raman measurements were made with SERS effect, on the cells, analizing the characteristic spectra of living cells after 0, 12 and 24 hours and the results obtained were contrasted with flow cytometry and fluorescence techniques. All the evaluated data were recorded in an Excel database and analyzed using the IBM® SPSS® Statistics 22.0.0 and Stata 11 software.

Results: The extracts studied, showed little toxicity on the hDPSC cells, in all concentrations and times. These results coincided with those found with fluorescence and cytometry techniques.

Conclusions: The extracts of the plants Ilex Guayusa and piper marginatum showed, in general, little cytotoxicity, suggesting that they are safe and that they could be used in the future to be applied on enamel and dentine for remineralization purposes. The Raman technique allowed the evaluation of cell viability against the extracts of these plants, which let its reliable use especially in toxicological studies in tissues of the oral cavity.

This abstract is based on research that was funded entirely or partially by an outside source:
COLCIENCIAS FP44842-058-2015

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE