Title: 2780 - Gender-specific Differences in the Salivary Microbiome of Caries-active Children
Stephanie Ortiz (Presenter)
Oregon Health & Science University
Anne Purcell, Oregon Health & Science University
Claudia Lyashenko, Oregon Health & Science University
Kareem Raslan, Oregon Health & Science University
Elisa Herrman, Oregon Health & Science University
Elizabeth Palmer, Oregon Health & Science University
Anna Forsyth, Oregon Health & Science University
Dongseok Choi, Oregon Health & Science University
Tom Maier, Oregon Health & Science University
Curt Machida, Oregon Health & Science University
Objectives: Dental caries represents one of the most common chronic diseases found in young children, and is a multi-factorial disease involving complex interactions of microbiological, genetic and socioeconomic risk factors. While women exhibit higher caries incidence than men, it is unclear if this disparity can be extended to children. The purpose of this study is to determine gender-specific differences in the salivary microbiome within caries-active children.
Methods: Saliva specimens were collected from 85 children (boys:41; girls:44) between the ages of 2-14 years. Microbial DNA was isolated using the QIAsymphony isolation robot, and then subjected to PCR amplification using V3-V4 16S rDNA-specific primers and next generation sequencing (NGS) with high-throughput Illumina sequencing. Oral microbiota libraries and profiles were generated by the Forsyth Institute, Cambridge, MA and subjected to further biostatistical analyses at OHSU.
Results: Significant differences in oral microbiota were found between caries-active boys versus girls. Moraxella genus probe 2, Streptococcus genus probe 2, Neisseria flavescens, Rothia aeria, and Haemophilus pittmaniae were found at significantly higher levels in caries-active boys than girls, exhibiting 171-, 26.4-, 20.7-, 3.9- and 3.8-fold differences, respectively. Alternately, Lactococcus lactis, Selenomonas species HOT 126, Actinobaculum species HOT 183, Lachnoanaerobaculum species HOT 083, Prevotella species HOT 315, Veillonella parvula, and Alloprevotella species HOT 473 were found at significantly higher levels in caries-active girls than boys, exhibiting 92.2-, 21.5-, 9.8-, 8.9-, 7.3-, 5.4- and 4.4-fold differences, respectively.
Conclusions: In the current literature, the primary microbial genera associated with caries in young children includes Actinobaculum, Atopobium, Aggregatibacter, and Streptococcus. In our studies, Actinobaculum, Veillonella parvula, and the acid-generating Lactococcus lactis – all microorganisms associated with dental caries – are found in much higher prevalence in caries-active girls than boys, indicating that these microorganisms may play a more significant role in girls to shape the cariogenic microbial environment. Supported by NIDCR R15DE024317 (CM).
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE