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Title: 2722 - Pre-treatment Molecular Detection of Periodontopathogens in Systemically Compromised Periodontitis Patients

Authors:

Andrea Escalante Herrera (Presenter)
Pontificia Universidad Javeriana

Fredy Gamboa, Pontificia Universidad Javeriana
Dabeiba Garcia, Pontificia Universidad Javeriana
Daniela Andrade, Pontificia Universidad Javeriana
Laura Rodriguez, Pontificia Universidad Javeriana
Laura Ruiz, Pontificia Universidad Javeriana

Abstract:

Objectives: To determine the presence and quantity of Porphyromonas gingivalis, Prevotella intermedia and Aggregatibacter actinomycetemcomitans in gingival sulcus and bloodstream in diabetic and hypertensive patients with a diagnosis of chronic periodontitis, prior to periodontal treatment.

Methods: Prior ethics committee approval and signing of informed consent, samples of gingival sulcus and bloodstream were taken from 40 patients before periodontal treatment, distributed equally in 4 groups based on the systemic and periodontal condition as follows: group 1; healthy systemically and periodontally, group 2; systemically healthy and chronic periodontitis, group 3; hypertension and chronic periodontitis, and group 4; type II Diabetes mellitus and chronic periodontitis. DNA from Porphyromonas gingivalis, Prevotella intermedia and Aggregatibacter actinomycetemcomitans was identified and quantified through the real-time polymerase chain reaction (PCR-TR) technique and descriptive analysis between groups was performed using the Chi square test and analysis of correlation between gingival sulcus and bloodstream using the Pearson coefficient.

Results: A predominance in the presence of Porphyromonas gingivalis in gingival sulcus was observed in patients diagnosed with chronic periodontitis, having a higher frequency in group 3. However, when observing the quantification of this microorganism, it was statistically significantly higher (p <0.038) in group 2. Additionally, when correlating these findings with the presence of microorganisms in the bloodstream, Porphyromonas gingivalis was observed in 3% (1/30) of patients with chronic periodontitis, with no statistical significance (p <0.70).

Conclusions: The results of the present study demonstrate the presence of Porphyromonas gingivalis and Prevotella intermedia in gingival sulcus both in systemically healthy patients and in patients with arterial hypertension and / or Diabetes mellitus with chronic periodontitis, with a higher tendency of Porphyromonas gingivalis in patients with hypertension. There is no presence of Porphyromonas gingivalis, Prevotella intermedia, Aggregatibacter actinomycetemcomitans in the bloodstream of patients with hypertension and controlled Diabetes mellitus, prior to periodontal treatment.

Table(s):

Variables Group 1
n=10
Total(%)
Group 2
n=10
Total(%)
Group 3
n=10
Total(%)
Group 4
n=10
Total(%)
Females 3(30) 5(50) 9(90) 5(50)
Males 7(70) 5(50) 1(10) 5(50)
Age† 33,2±11,5* 42,6±13,9 59±12,0 65,7±5,9
Gingival bleeding† 16,6±16,0* 67,9±36,9 69,4±30,8 82,0±25,6
Periodontal probing depth† 1,9±0,1* 2,6±0,5 2,5±0,2 2,8±0,6
Clinical attachment level† 1,0±0,4* 2,1±0,6 2,5±0,7 3,3±0,6
Amount of teeth in mouth† 27,2±2 24,2±3,5 19,6±5,2 20±3,1
Affected teeth† N.A. 9,5±5,5 9,5±5,6 7,8±5,0
Periodontal probing depth of affected teeth N.A. 4,4±0,4 4,3±0,3 4,3±0,4
Variable Group 1
H + H
n=10
Group 2
H + P
n=10
Group 3
AH + P
n=10
Group 4
MD + P
n=10
Porphyromonas gingivalis
Presence
0/10 5/10 7/10* 4/10
Porphyromonas gingivalis
Quantity‡
N.A. 152485 (95-892338)* 34486 (262-311451) 3166 (136-15282)
Prevotella intermedia
Presence
1/10 3/10 1/10 3/10
Prevotella intermedia Quantity‡ 24 (23-26) 65 (7-200) 314 (100-528) 4376 (33-12263)
Aggregatibacter actinomycetemcomitans
Presence
0/10 1/10 1/10 0/10
Aggregatibacter actinomycetemcomitans
Quantity‡
N.A. 2274 (1503-3045) 1095 (895-1295) N.A.
P. gingivalis + P. intermedia
Presence
0/10 3/10 1/10 2/10
P. gingivalis + A. actinomycetemcomitans
Presence
0/10 1/10 0/10 0/10

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE

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